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Simple, scalable proteomic imaging for high-dimensional ...
Combined measurement of diverse molecular and anatomical traits that span multiple levels remains a major challenge in biology. Here, we introduce a simple method that enables proteomic imaging for scalable, integrated, high-dimensional phenotyping of both animal tissues and human clinical samples.

Simple, Scalable Proteomic Imaging for High-Dimensional ...
Proteomic imaging requires (1) high preservation of endogenous biomolecules and their antigenicity, (2) high structural integrity, and (3) minimal tissue damage during repeated cycles of destaining, labeling, and imaging processes.

The Gain in Brain: Novel Imaging Techniques and ...
The pioneering field of ultrastructural multiplexed proteomic imaging now includes a number of high resolution methods, such as array tomography, stimulated emission depletion microscopy, stochastic optical reconstruction microscopy and automated transmission electron microscopy, which allow a detailed molecular characterization of individual synapses and subsynaptic structures within brain tissues for the first time.

Perkin Elmer ProXpress Proteomic 2D Imaging System ...
This Perkin Elmer ProXpress Proteomic 2D Imaging System. System features include: Multi-color: Infinite sample wavelength selection in the VIS and UV range; multi-color detection, 6-position filter wheels – detect almost anything! High sensitivity: ≤1 ng protein, SYPRO® Ruby stained; ≤0.3 ng protein, Alexa™ 488 stained – eliminate or reduce the possibility of missing important data ...

Perkin Elmer ProXPRESS 2D Proteomic Imaging System - Cal-L ...
The ProXPRESS™ 2D Proteomic Imaging System will reveal proteins you otherwise wouldn’t even know were there. It’s a high-speed CCD-based imager with superior sensitivity, resolution and a wide dynamic range. ProXPRESS 2D provides accurate, reproducible detection and quantitation of low abundance proteins— so you never have to wonder if ...

Improved Imaging, Proteomics-Style | The Scientist Magazine®
Home; Archive; May 2004; Tools and Technology; Improved Imaging, Proteomics-Style Courtesy of PerkinElmer Life and Analytical SciencesBoston-based PerkinElmer Life ...

Simple, Scalable Proteomic Imaging for High-Dimensional ...
Proteomic imaging requires (1) high preservation of endogenous biomolecules and their antigenicity, (2) high struc- tural integrity, and (3) minimal tissue damage during repeated cycles of destaining, labeling, and imaging processes. We first tested whether endogenous biomolecules are well preserved by measuring protein loss after clearing (see

NIH VideoCast - Proteomic Imaging of Endothelium and its ...
This type of "proteomic imaging of the vascular biomarker space including its in vivo accessible target space and transvascular pumping space" may bode well for application to cancer and many other diseases. Dr. Jan E. Schnitzer is the Scientific Director of the Sidney Kimmel Cancer Center, located in San Diego. The Sidney Kimmel Cancer Center ...

JCM | Free Full-Text | Connecting Histopathology Imaging ...
Proteomics data encode molecular features of diagnostic value and accurately reflect key underlying biological mechanisms in cancers. Histopathology imaging is a well-established clinical approach to cancer diagnosis. The predictive relationship between large-scale proteomics and H&E-stained histopathology images remains largely uncharacterized.

SWITCH protocol | Abcam
SWITCH protocol Quickly process and clear tissues for repeated labeling and multiplexed imaging. SWITCH (system-wide control of interaction time and kinetics of chemicals) is a simple, scalable, and generalizable tissue-processing method for proteomic imaging of intact biological systems.

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